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A DNA profile of species-specific microsatellite markers is obtained from the offspring, potential dam, and potential sire(s), and parentage analysis is performed using this data. In this process, a computer program compares the DNA profile of the offspring to the profiles of the presumed parents. Candidate sires and dams may either qualify or be excluded as parents based on whether they share microsatellite marker alleles with the offspring. A parentage analyst then reviews the results and sends the final report. If a listed parent or parents are excluded, additional analysis is performed, including retesting of samples and the possible use of additional DNA markers to confirm the exclusion.
You can read more about the process of DNA parentage analysis HERE.
In bison, the following microsatellite markers are reported:
BM1225
BMS410
BMS527
INRA194
RM372
SPS115
TGLA44
BM1706
BM17132
BM720
BMC4214
BMS1001
BMS1117
BMS1862
BMS2639